Analytical Training Module
These training modules are aimed to provide an insight into systematic approach for the extraction, fractionation, isolation, and structural characterization of phytochemical constituents present in medicinal plants by using sophisticated analytical instruments. The participants will be introduced to the different extraction and chromatographic techniques, the principle of the instruments that are used for screening of bioactive compounds, qualitative and quantitative analysis of potential bioactive compounds, etc. The participants will be provided with hands on experience for preparing plant extracts, fractionation, and isolation of compounds followed by sample preparation techniques. The participants will also have a chance to interact with experts and analyse their own samples (with prior approval).
Objectives:
- • About 40% theory and 60% practical sessions as per course module
- • Hands on training
- • Job oriented curriculum
- • Understanding basic principles
- • Interactive session
- • Brief Hands-on practical exposure on the state-of-the-art equipment’s
Training Program Modules
Module-1
Introduction to Pharmacognosy and understanding the traditional method of Drug Screening
General introduction to Pharmacognosy, plant diversity, plant cultivation, important terms used, plant collection and processing, raw material standardization, secondary metabolites, classification of phytochemicals from plant sources and understanding the traditional method of drug screening, etc.
Module-2
Basics of different extraction techniques and different chromatographic techniques
General introduction to different extraction techniques, bioactivity guided fractionation, qualitative test for different groups of phytochemicals, quantitative test of bioactive compounds, introduction to different chromatographic techniques, hands-on to different extraction instruments like soxhlet apparatus, microwave extraction, microwave extraction for flavors and fragrances, ultrasonic assisted extraction, hands-on to different chromatographic techniques like column chromatography, thin layer chromatography, estimation of qualitative profile, isolation of bioactive compounds, etc.
Module-3
Basics of spectroscopic instruments, qualitative and quantitative analysis using UV and FTIR
General introduction to different spectroscopic techniques, principle of UV and FTIR, qualitative test of phytochemicals using UV and FTIR, quantitative estimation of bioactive molecules using UV and FTIR, Characterization of isolated compounds and purity analysis of the compounds, hands-on to UV and FTIR for qualitative and quantitative analysis, etc.
Module-4
Basics of chromatographic instruments, qualitative and quantitative analysis using TLC, HPTLC, HPLC, UPLC, GC
General introduction to different chromatographic techniques, principle of the chromatographic techniques, qualitative test of phytochemicals using chromatographic instruments, quantitative estimation of bioactive molecules using chromatographic instruments, primary standards, secondary standards, different assay techniques, brief summary of method development and validation, ICH guidelines, purity analysis of the compounds, assay of formulations, calculations and reporting, hands-on to the chromatographic instruments for qualitative and quantitative analysis, etc.
Module-5
Basics of mass spectroscopy, qualitative and quantitative analysis using LC-HRMS and GC-MS
General introduction to mass spectroscopy, principle of the mass spectroscopy, qualitative screening of crude extracts, structural characterization of compounds, library search, quantitative analysis of bioactive compounds, and pesticide residue estimation, etc.
Module-6
Calibration of analytical instruments
General introduction to the need of calibration, calibration procedure of the analytical instruments, daily and half-yearly calibration instruments methods, etc.
Module-7
Documentation
Preparation of calculation sheet, preparation of SOP, Preparation of analysis protocols, approvals, maintenance of calibration records, keeping records of sample receiving and dispatch, and preparation of reports, etc.
Evaluation of trainees and certification
Evaluation of the training will be done and certificate will be issued to the successful candidates for the course..
